System overview

• Flexible platform for trapping and tracking of nanoparticles for measurements from single-molecules to entire cells
• Compatible with inverted microscopes for combined experiments, e.g.,
  • Carl Zeiss Axiovert 200, Axio Observer
  • Nikon TE 2000 & Eclipse Ti/Ti2
  • Olympus IX70/71/73
  • Leica DMi8
• Compatible with all major light microscopy techniques such as DIC and fluorescence
• Spectral range of 400-900 nm for undisturbed fluorescence imaging
• Optical components for trapping are optimized for 1064 nm wavelength
• Automated calibration for enhanced productivity
• All major components are motorized and computer-controlled
• No safety goggles needed (Class 1 laser) and no alignment by hand
• Optional external standard modules such as confocal units, CCD cameras, detectors

NanoTracker™ 2 head

• Closed head design prevents stray light and airborne noise for noise-free measurements
• Liquid-safe, robust and drift-minimized design for highest stability
• Optional sample positioning piezo stage with up to 100 ×100 ×100 μm³ travel range and closed-loop control for accurate trap calibration, fast vertical scanning and experimental flexibility
• Software controlled motorized precision stage with 20 ×20 mm² travel range
• Oil or water immersion lenses for high power or low spherical aberration for advanced trapping applications
• Four sensitive InGaAs photo detectors with up to 3.5 MHz bandwidth (16 bit sampling)
• Decoupled detection of XY and Z for optimized Z sensitivity
• Precise focus adjustment via software

NanoTracker™ 2 sample holder options

• Standard slides, cover glasses or Petri dishes can be used
• Multichannel LaminarFlowCell (LFC™) is a coverslip-based fluid cell with laminar flow perfusion capabilities for single-molecule applications
• Living cell studies with temperature control (ambient to 60 °C), perfusion and gas flow (CO2) for 35 mm Petri dishes with glass bottom from Wilco, BD, and Corning

All fluid cells and temperature control options

NanoTracker™ 2 laser unit

• Ultra-stable custom-designed laser (1064 nm)
• < 0.5 % intensity stability
• 3, 5 and 10 W laser power
• Class 1 laser certification

NanoTracker™ 2 steering unit

• True 3D continuous beam steering through the full field of view
• One or two beams with adjustable power distribution
• One beam with XY steering by advanced piezo mirrors or AOD, and Z steering
• 500 μsec response time for steering unit

NanoTracker™ 2 controller

• State-of-the-art controller with lowest noise levels
• 60 MHz sample rate with 16 bit for XY and Z detection
• Signal Access Module (SAM) with more than 20 input/output channels
• TTL access and power supply for external equipment
• High-speed Ethernet link and intelligent grounding concept for maximum bandwidth and performance

NanoTracker™ 2 software

• Easy to use Java™-based user interface for intuitive instrument control
• Point and Trap™ beam steering
• Automated force versus displacement calibration
• JPK‘s proven DirectOverlay™ functionality for precise matching of trap and sample position
• Embedded camera control for high-end EM-CCD‘s
• Advanced oscilloscope functionality and online measurement of distances, cross sections, and many more
• Powerful force spectroscopy with the new RampDesigner™ for advanced force clamp or force ramp experiments
• JPK’s ExperimentPlanner™ for easy design of advanced experimental routines
• Advanced and high-speed batch processing force curve analysis with huge number of fitting models including WLC, FJC or step fitting algorithms
• Complete environmental control via software
• User-programmable software for advanced experiments
• User-definable shortcut buttons
• Traps, motor and shutter steering control via gamepad

Optional modular items for enhanced flexibility

• Single-beam/dual-beam configuration
• Detection unit: equipped as single-beam or dual-beam
• AOD option for faster beam steering and optimized trap multiplexing
• Closed-loop piezo stage configurations: 3-axes, 1-axis (only Z) or none
• Axial trap control for independent focus of traps and microscope
• Lasers with different output power
• Choice of microscope objectives: magnification, immersion medium, TIRF
• MagneticTwister™ add-on