System overview
- Flexible platform for trapping and tracking of nanoparticles for measurements from single-molecules to entire cells
- Compatible with inverted microscopes for combined experiments, e.g.,
- Carl Zeiss Axiovert 200, Axio Observer
- Nikon TE 2000 & Eclipse Ti/Ti2
- Olympus IX70/71/73
- Leica DMi8
- Compatible with all major light microscopy techniques such as DIC and fluorescence
- Spectral range of 400-900 nm for undisturbed fluorescence imaging
- Optical components for trapping are optimized for 1064 nm wavelength
- Automated calibration for enhanced productivity
- All major components are motorized and computer-controlled
- No safety goggles needed (Class 1 laser) and no alignment by hand
- Optional external standard modules such as confocal units, CCD cameras, detectors
NanoTracker™ 2 head
- Closed head design prevents stray light and airborne noise for noise-free measurements
- Liquid-safe, robust and drift-minimized design for highest stability
- Optional sample positioning piezo stage with up to 100 ×100 ×100 μm³ travel range and closed-loop control for accurate trap calibration, fast vertical scanning and experimental flexibility
- Software controlled motorized precision stage with 20 ×20 mm² travel range
- Oil or water immersion lenses for high power or low spherical aberration for advanced trapping applications
- Four sensitive InGaAs photo detectors with up to 3.5 MHz bandwidth (16 bit sampling)
- Decoupled detection of XY and Z for optimized Z sensitivity
- Precise focus adjustment via software
NanoTracker™ 2 sample holder options
- Standard slides, cover glasses or Petri dishes can be used
- Multichannel LaminarFlowCell (LFC™) is a coverslip-based fluid cell with laminar flow perfusion capabilities for single-molecule applications
- Living cell studies with temperature control (ambient to 60 °C), perfusion and gas flow (CO2) for 35 mm Petri dishes with glass bottom from Wilco, BD, and Corning
All fluid cells and temperature control options
NanoTracker™ 2 laser unit
- Ultra-stable custom-designed laser (1064 nm)
- < 0.5 % intensity stability
- 3, 5 and 10 W laser power
- Class 1 laser certification
NanoTracker™ 2 steering unit
- True 3D continuous beam steering through the full field of view
- One or two beams with adjustable power distribution
- One beam with XY steering by advanced piezo mirrors or AOD, and Z steering
- 500 μsec response time for steering unit
NanoTracker™ 2 controller
- State-of-the-art controller with lowest noise levels
- 60 MHz sample rate with 16 bit for XY and Z detection
- Signal Access Module (SAM) with more than 20 input/output channels
- TTL access and power supply for external equipment
- High-speed Ethernet link and intelligent grounding concept for maximum bandwidth and performance
NanoTracker™ 2 software
- Easy to use Java™-based user interface for intuitive instrument control
- Point and Trap™ beam steering
- Automated force versus displacement calibration
- JPK‘s proven DirectOverlay™ functionality for precise matching of trap and sample position
- Embedded camera control for high-end EM-CCD‘s
- Advanced oscilloscope functionality and online measurement of distances, cross sections, and many more
- Powerful force spectroscopy with the new RampDesigner™ for advanced force clamp or force ramp experiments
- JPK’s ExperimentPlanner™ for easy design of advanced experimental routines
- Advanced and high-speed batch processing force curve analysis with huge number of fitting models including WLC, FJC or step fitting algorithms
- Complete environmental control via software
- User-programmable software for advanced experiments
- User-definable shortcut buttons
- Traps, motor and shutter steering control via gamepad
Optional modular items for enhanced flexibility
- Single-beam/dual-beam configuration
- Detection unit: equipped as single-beam or dual-beam
- AOD option for faster beam steering and optimized trap multiplexing
- Closed-loop piezo stage configurations: 3-axes, 1-axis (only Z) or none
- Axial trap control for independent focus of traps and microscope
- Lasers with different output power
- Choice of microscope objectives: magnification, immersion medium, TIRF
- MagneticTwister™ add-on
Instrument modes
- 3D particle tracking
- Point and Trap™ for easy trap positioning
- Online calibration based on power spectrum
- Advanced Force Spectroscopy including Force Clamp and Force Ramp with the new JPK RampDesigner™
- Active and passive force mapping
- Micro-rheology mode
- Optical sorting
- Trap oscillation
- Multiplexing for 3D trap arrays
- Line traps & circular traps
- Microfluidics control
- Nanoassembly
- Optical z-stacking
Advanced optical microscopy modes
- Brightfield transmission illumination (standard)
- Differential Interference Contrast (DIC) in transmission (standard)
- Epi-fluorescence microscopy (standard)
- Raman spectroscopy
- TIRF microscopy
- Confocal microscopy
- FRET microscopy and more
Applications
Single-molecules & biopolymers
- Intra-molecular elasticity & protein folding dynamics
- Motor protein tracking
- DNA/RNA mechanics
- Protein-DNA binding
- Nanopores & 3D polymer network probing
Cell biology applications
- Membrane organization (e.g., lipid rafts)
- Trans-membrane processes, trafficking
- Intracellular forces
- Receptor-ligand experiments
- Cell mechanics and cell motility
- Membrane tether dynamics
- Micro-rheology of cells and gels
Cell-particle interaction and infection studies
- Tracking of pathogen-host interaction and escape forces
- Bacterial and virus adhesion forces
- Local gene or drug delivery
- Entrance mechanism studies
- Nanotoxicity & endocytosis studies
Advanced measurements
- Complex optical trap geometries
- Optical guiding & artificial crystal building
- Local field enhancement & Raman/SERS applications
- Brownian motion tracking, Photonic Force Microscopy (PFM)
- Colloidal and polymer meshworks force probing
- Video particle tracking and optical spectroscopy