技术参数 - NanoTracker™ 2 - JPK BioAFM

System overview

Flexible platform for trapping and tracking of nanoparticles for measurements from single-molecules to entire cells
Compatible with inverted microscopes for combined experiments, e.g.,
- Carl Zeiss Axiovert 200, Axio Observer
- Nikon TE 2000 & Eclipse Ti/Ti2
- Olympus IX70/71/73
- Leica DMi8
Compatible with all major light microscopy techniques such as DIC and fluorescence
Spectral range of 400-900 nm for undisturbed fluorescence imaging
Optical components for trapping are optimized for 1064 nm wavelength
Automated calibration for enhanced productivity
All major components are motorized and computer-controlled
No safety goggles needed (Class 1 laser) and no alignment by hand
Optional external standard modules such as confocal units, CCD cameras, detectors

Closed head design prevents stray light and airborne noise for noise-free measurements
Liquid-safe, robust and drift-minimized design for highest stability
Optional sample positioning piezo stage with up to 100 ×100 ×100 μm³ travel range and closed-loop control for accurate trap calibration, fast vertical scanning and experimental flexibility
Software controlled motorized precision stage with 20 ×20 mm² travel range
Oil or water immersion lenses for high power or low spherical aberration for advanced trapping applications
Four sensitive InGaAs photo detectors with up to 3.5 MHz bandwidth (16 bit sampling)
Decoupled detection of XY and Z for optimized Z sensitivity
Precise focus adjustment via software

Standard slides, cover glasses or Petri dishes can be used
Multichannel LaminarFlowCell (LFC™) is a coverslip-based fluid cell with laminar flow perfusion capabilities for single-molecule applications
Living cell studies with temperature control (ambient to 60 °C), perfusion and gas flow (CO2) for 35 mm Petri dishes with glass bottom from Wilco, BD, and Corning

State-of-the-art controller with lowest noise levels
60 MHz sample rate with 16 bit for XY and Z detection
Signal Access Module (SAM) with more than 20 input/output channels
TTL access and power supply for external equipment
High-speed Ethernet link and intelligent grounding concept for maximum bandwidth and performance

Easy to use Java™-based user interface for intuitive instrument control
Point and Trap™ beam steering
Automated force versus displacement calibration
JPK‘s proven DirectOverlay™ functionality for precise matching of trap and sample position
Embedded camera control for high-end EM-CCD‘s
Advanced oscilloscope functionality and online measurement of distances, cross sections, and many more
Powerful force spectroscopy with the new RampDesigner™ for advanced force clamp or force ramp experiments
JPK’s ExperimentPlanner™ for easy design of advanced experimental routines
Advanced and high-speed batch processing force curve analysis with huge number of fitting models including WLC, FJC or step fitting algorithms
Complete environmental control via software
User-programmable software for advanced experiments
User-definable shortcut buttons
Traps, motor and shutter steering control via gamepad

3D particle tracking
Point and Trap™ for easy trap positioning
Online calibration based on power spectrum
Advanced Force Spectroscopy including Force Clamp and Force Ramp with the new JPK RampDesigner™
Active and passive force mapping
Micro-rheology mode
Optical sorting
Trap oscillation
Multiplexing for 3D trap arrays
Line traps & circular traps
Microfluidics control
Nanoassembly
Optical z-stacking